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Antibody Purification and Labeling

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  • 2019/05/07
    We launched Antibody sequencing service.

Antibody sequencing

We provide sequencing service of valiable region of mouse monoclonal antibody. The antibody sequence information is essential for antibody engineering including humanization and drug development.
We analyze the sequence not only for hybridoma we have established as service, but also for hybridomas you already have.

  Service contents Deriverables Standard time period Price
Antibody sequencing ・Preparation of total RNA from mouse hybridoma.
・Synthesis of cDNA
・PCR amplification of variable region with primer mix specific for heavy chain and light chain each Sequencing of PCR amplified fragments
・Isolation of leader sequence by 5’ RACE PCR
・Identification of sequences from leader sequence to variable region

※Sample condition to be provided
Hybridoma (mouse) cell pellet (1 x 106 cells or more)
Antibody isotype should be IgG, and light chain should be κ chain.
Sequence data from leader to variable region in heavy chain and light chain
2 months JPY 500,000/
clone
Options Culture of hybridoma
-
-
please inquire
Isotyping of antibody
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-
JPY 90,000/
antibody

Antibody purification and labeling

  Content of work Quantity Price
Purification of Polyclonal Antibodies ・Affinity purification from anti-sera.
・ELISA (titer) analysis.
20mL~ JPY120,000~
Purification of Monoclonal Antibodies ・Hybridoma culture
・Purification by ProteinG column chromatography
・ELISA (titer) analysis
~100mL JPY200,000~
Antibody labeling Biotin ~500μg JPY70,000
Horseradish peroxidase (HRP) ~500μg JPY80,000
Alkaline phosphatase (ALP) ~500μg JPY100,000
Fluorescein isothiocyanate (FITC) ~500μg JPY100,000

Large scale preparation of monoclonal antibodies

  Contents Scale of culture Period Price
High density cell culture High density culture of hybridoma in serum free medium (SFM)

Collection of cultured supernatant
1 L 1.5 months JPY250,000
5L 2 months JPY550,000
High density cell culture and IgG purification High density culture of hybridoma in serum free medium (SFM) Collection of cultured supernatant

Purification by ProteinG column chromatography

Measurement of protein concentration
1 L 2.5 months JPY550,000
5L 3.5 months JPY1,200,000

※Please inquire about other custom services and large scale services.

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