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TG Resource Bank®

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TG Resource Bank®

TG Resource Bank®

The exchangeable gene trap method is one of our company's basic technologies. Developed by Professor Ken-ichi Yamamura and others at Kumamoto University, it has been patented in the United States, Europe and elsewhere. Using this method, we have assiduously and extensively trapped mouse genes to build the TG Resource Bank®.
The TG Resource Bank® is a library composed of approximately 750 strains obtained by the gene trap method. You can choose the strain and delivery date that suits your purposes and budget.

We produce and retain 750 strains of mice and 2000 strains of ES cells.
<Examples of Trapped Genes>
Elovl6/Tet2/Glrx5/Dst/Acot8/Metap1/B3bp/Rnf111/
Nup210/Atf5/ Ube2j2/Rassf3/Hip1/Smad2/Jarid2/
Aldoa …etc
  • Elovl6 Knockout Mice(498KB)

How the TG Resource Bank® Operates
Our company sells “the right to use" mice. That is, as a rule, we provide pairs of heterozygous mice. Following purchase, you may breed the pair freely. However, please do not transfer them to a third party. Moreover, should the colony become extinct, you can obtain frozen embryos which our company keeps as backup for the pair.

TG Resource Bank® Mouse Library
This library consists of established strains of heterozygous mice. Breeding pairs are delivered within a few months of the customer's order.
  • Mouse Library (750 strains) PHP
  • Mouse Library (750 strains) EXCEL
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About the Exchangeable Gene Trap Method

1. Production of Trapped ES Clones
Using electroporation, a trap vector is introduced into a mouse ES cell (TT2). The vector is randomly inserted into the mouse genome, but only when it is inserted into the gene region can a reporter gene be expressed by an endogenous promoter and a clone be obtained. Through insertion of the vector, expression of the genes immediately below the insertion site is inhibited.
Structure of Trap Vector

2. Analysis of Sequence of Trapped Genes
Using the 5'-RACE method, information is obtained on the cDNA sequence downstream from the insertion site. Based on this sequential information, the trapped gene is identified. We also confirm that only one copy of the vector has been inserted (and thus that only one gene has been trapped).Analysis of Sequence of Trapped Genes

3. Mouse Production
Using the ES cell into which the vector is inserted, a chimeric mouse is produced by the aggregation method. Germline transmission is confirmed and F1 heterozygous mice are then produced.

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An Application Using an ES Clone Made with the Exchangeable Gene Trap Method

Method of Producing a Humanized Mouse
1. A clone with an exchangeable trap vector inserted into the gene to be humanized is obtained from the TG Resource Bank®

2. A target vector like that shown in the diagram is constructed. The target vector is given a linear-chain form and, together with Cre recombinase, is inserted into an ES cell by electroporation.
Insertion of expression cassette 3. A chimeric mouse is produced using ES cell lines selected and recombined with the drug-resistant gene (in this case, puromycin) contained in the target vector.

4. A specific gene of the homologous genetically engineered mouse obtained from the chimeric mouse then becomes the mouse that is to be humanized.

5. This mouse is crossed with an Flp mouse, after which a humanized conditional knockout mouse with a deleted gene can be produced from just a specific organ.Deletion of introduced cassette

< References >
■PLOS GENETICS. 2013
Ectopic Expression of Ptf1a Induces Spinal Defects,Urogenital Defects, and Anorectal Malformations in Danforth's Short Tail Mice
Kei Semba, Kimi Araki, Ken-ichirou Matsumoto, Hiroko Suda, Takashi Ando, Akira Sei, Hiroshi Mizuta, Katsumasa Takagi, Mai Nakahara, Mayumi Muta, Gen Yamada, Naomi Nakagata, Aritoshi Iida, Shiro Ikegawa, Yusuke Nakamura, Masatake Araki, Kuniya Abe, Ken-ichi Yamamura

■Cell Mol Biol (Noisy-le-grand). 1999 Jul;45(5):737-50
Exchangeable gene trap using the Cre/mutated lox system
Araki K, Imaizumi T, Sekimoto T, Yoshinobu K, Yoshimuta J, Akizuki M, Miura K, Araki M, Yamamura K

■Folia Pharmacol. Jpn 2007 129:337-342
Genetically disrupted mouse production technology based on the gene trap method
Ken-ichi Yamamura

■Cancer Sci. 2008 Jan;99(1):1-6. review
Gene trap mutagenesis in mice: new perspectives and tools in cancer research
Yamamura K, Araki K.

<Countries with Patents for the Exchangeable Gene Trap Method>
United States (Patent No. US 7,312,075)
Europe: (Patent No. EP1201759)
Australia (Patent No. AU 778,719)
China (Patent No. ZL00812904.5)

TG Resource Bank® Mouse Library
This library consists of established strains of heterozygous mice. Breeding pairs are delivered within a few months of the customer's order.
  • Mouse Library (750 strains) PHP
  • Mouse Library (750 strains) EXCEL
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Comparison of Knockout Mouse Supply Services

  TransGenic Inc. Deltagen Inc.
TG Resource Bank® Custom production of knockout mice DeltaOne™
Trapped genes Extensively, randomly trapped genes
(Approx. 2,700 genes)
The genes desired by customers Genes targeted in drug discovery and deemed highly useful
(Approx. 900 genes)
Production method Exchangeable gene trap method Gene targeting method Gene targeting method
ES cells used TT2 Derived from C57BL/6
(Can also be derived from 129/sv, BALB/c)
Derived from 129
Delivery time Mice: Approx. 3 months Mice: Approx. 1 year Mice: Approx. 3 months
Embryos: Approx. 1 month
Rights Right of use is licensed Rights belong to customer Right of use is licensed

*Please inquire about the details of each service.

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