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Custom Production of Genetically Engineered Mice

Cryopreservation of embryo and sperm

Trans Genic Inc. offers various services for mouse production and strain preservation, based on our wide experience in embryo manipulation and reproductive biology through production of genetically engineered mice.

Outline of cryopreservation

Process Contents Price
Production of frozen embryos Sperms, isolated from your male mice, are in vitro fertilized with eggs from wild-type C57BL/6N mice, and fertilized eggs are cryopreserved. Viability and ability of normal development are confirmed by using a part of frozen embryos. JPY220,000
Production of frozen sperms Sperms, isolated from your male mice are isolated and cryopreserved.
Approximately 10 straws (10μL) are producted.
JPY100,000
Recovery of mice from frozen embryos Your frozen embryos are transplanted into 2 – 3 recipient mice to recover individuals. Pups are housed until 8 weeks old and genotyped by PCR. JPY300,000
Recovery of mice from frozen sperm Your frozen sperm are used for in vitro fertilization (IVF) to recover individuals. Pups are housed until 8 weeks old and genotyped by PCR. JPY400,000
Transportation of frozen embryo Samples are delivered to your designated facility in Dry Shipper. JPY5,000
Storage of cryopreserved embryos and sperms Cryopreserved embryos and sperms are stored in liquid nitrogen tank for 1 year.
*Price for 1 strain.
JPY40,000
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