2009 / 5 / 15   We renewed a list of gene knockout mice (346 lines updated).

2007 / 7 / 30   We renewed a list of gene knockout Mice.
 
 

+ All lines in TG Resource Bank, using exchangeable gene-trap method.

+ Unlike gene targeting method, exchangeable gene-trap method destroys genes at random.
+ Only genes expressed in ES cells are trapped.
+ Trapped mouse genes are exchangeable with other genes such as human genes and genes containing point mutation.
+ Expression analysis of the trapped genes is easily performed by X-gal staining
+ Our knockout mice library consists of established heterozygous mice. We can not assure if homozygous mice bred from them are also viable.
+ The trapped genes are identified by insertion sites of their trap-vector, not by gene expression.

Reference:
Cell. Mol. Biol. (Noisy-le-grand). 1999 Jul; 45(5): 737-50.
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10512203

We sell “use right” of our mice and basically provide a breeding pair of heterozygous mice. Feel free to breed the pair after your purchase but refrain from transferring them to the third party. In the case of extinction of the colony, you can still obtain frozen embryos of the pair preserved for backup.

(1) Mouse Strain Library
Our knockout mice library consists of strains of established heterozygous mice. Breeding pairs of the knockout mice will be delivered within a few months after your order.

Gene List of our Mouse Library > [HTML file]  [Excel file]  [Key word search]

(2) Knockout ES Strain Library
This library consists of ES strains of knockout mice which have not yet been established as heterozygous mice.

Gene List of our ES Library > [HTML file] [Excel file] [Key word search]

***The above HTML files show a part of the lists. To see the whole lists, please download the excel files.

Prices differ by mouse strains. Please feel free to contact us.

We use “exchangeable gene-trap method” to produce knockout mice. The following are the procedures.

(1) Cloning of ES cells inserted with a trap-vector
To make a large-scale knockout mouse library, we apply the gene-trap method to ES cells. In this approach, one type of promoterless reporter genes(*) downstream of splice acceptor sequence are introduced into ES cells (TT2 clone) by electroporation. The structure of a vector used to create our knockout mouse libraries is illustrated below.
(*)beta-galactosidase /neomycin-resistance fusion gene (beta-geo)

When a vector is inserted into an endogenous and functioning gene upstream of gene sequence, a fusion transcript (beta-geo) is generated. This brings about selection of trapped clones by G418 and functions of endogenous genes are disrupted.

(2) Identification of genes trapped by the vector
Trapped cDNA is obtained by rapid amplification of cDNA 5’-ends (5’-RACE). Trapped gene is presumably identified by its genomic region homological to the position in 5’-RACE sequence offered by public mouse genome databases. We use Southern blotting to assure that one copy of the trap vector is inserted into one mouse gene.

(3) Production of knockout mouse
To produce chimeric mouse, a trapped ES clone cell is integrated into an 8-cell embryo by aggregation method.