• 学会・論文情報

GANP®技術に関する新知見が「The Journal of Immunology」に掲載されました。

 このたび掲載されました論文は、熊本大学大学院 生命科学研究部 感染免疫学講座 免疫学分野 阪口薫雄教授らの研究グループの成果で、エイズウイルスの感染能力を消失させ破壊する酵素をGANP分子がウイルスの中枢へ運び込むことを発見したものです。 


GANP Interacts with APOBEC3G and Facilitates Its Encapsidation into the Virions To Reduce HIV-1 Infectivity.
Maeda K, Almofty SA, Singh SK, Eid MM, Shimoda M, Ikeda T, Koito A, Pham P, Goodman MF, Sakaguchi N.



Abstract
The ssDNA-dependent deoxycytidine deaminase apolipoprotein B mRNA-editing, enzyme-catalytic, polypeptide-like 3G (A3G) is a potent restrictive factor against HIV-1 virus lacking viral-encoded infectivity factor (Vif) in CD4+ T cells. A3G antiretroviral activity requires its encapsulation into HIV-1 virions. In this study, we show that germinal center-associated nuclear protein (GANP) is induced in activated CD4+ T cells and physically interacts with A3G. Overexpression of GANP augments the A3G encapsidation into the virion-like particles and ∆Vif HIV-1 virions. GANP is encapsidated in HIV-1 virion and modulates A3G packaging into the cores together with cellular RNAs, including 7SL RNA, and with unspliced HIV-1 genomic RNA. GANP upregulation leads to a significant increase in A3G-catalyzed G→A hypermutation in the viral genome and suppression of HIV-1 infectivity in a single-round viral infection assay. Conversely, GANP knockdown caused a marked increase in HIV-1 infectivity in a multiple-round infection assay. The data suggest that GANP is a cellular factor that facilitates A3G encapsidation into HIV-1 virions to inhibit viral infectivity.