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Custom Production of Genetically Engineered Mice

Production of Transgenic Mice

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We have started TRECK mouse production service.
TRECK system is a method to generate novel model mouse in which specific type(s) of cells can be eliminated in living mouse. In this system, transgenic mouse is constructed to express Diphtheria toxin receptor (DTR, hHB-EGF) under specific promoter of your interest. The cells expressing DTR is eliminated following administration of Diphtheria toxin to the mouse.
TRECK system can be useful to analyze the phenotype and regeneration after specific type of cells are eliminated in living mouse, and generate novel type of disease model mouse. This system was developed by Prof. Kenji Kohno in Nara Institute of Science and Technology (Nat. Biotechnol, 19; 746-750, 2001).
Please also see our Plasmid product section in this website.

Production of Transgenic Mice

Trans Genic Inc. offers contract transgenic mice production service.
We purify DNA fragment for microinjection from plasmid DNA provided by the customer. Then, purified DNA fragment is microinjected into 200 fertilized eggs of C57BL/6 mouse strain for producing founder mice (F0). Tail samples are obtained from weaned and used for genotyping PCR analysis to identify founder (F0) mice.
Additionally, Trans Genic Inc.also offers design and construction of transgenic vector, production of F1 mice, breeding, and cryopreservation of embryos and sperm.

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Outline of Production Work

Process

Standard Period

Price

1. Linearization and purification of plasmid DNA
2. Microinjection into 200 fertilized eggs (C57BL/6 mouse strain)
3. Housing of pups until 8 weeks old
About 4 month JPY700,000
4.Setting up the condition for genotyping PCR analysis
5. Collection of tissue samples and genotyping PCR to identify founder mice
1 month JPY200,000

* Founder (F0) mice are produced.
* If genotyping result shows no founder (F0) mice, fee for processes 4 and 5 is refunded.
* We also offer various optional services including design and construction of expression vector, production of F1 mice, and housing of transgenic mouse line (please see below).
* Cost for shipping and health status report is not included.

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Optional services

Process

Price

Additional microinjection into 100 fertilized eggs JPY200,000~
Design and construction of transgenic vector JPY100,000~
Production of F1 mice by in vitro fertilization (IVF) (from founder male)

* including housing until 8 weeks old and genotyping analysis
JPY350,000~
Production of next generation by natural mating (from founder male)

Mice are housed and bred to produce next generation (3 months) by using one shelf space exclusively for one strain in our SPF facility. Pups are housed until 8 weeks old and genotyped by PCR.

* Up to 10 cages can be placed on one shelf space.
* In case pups are not obtained, only JPY70,000 are charged for the fee of mating setup.
JPY200,000~
Cryopreservation of embryos (200 embryos) JPY220,000~
Cryopreservation of sperm (10 straws) JPY100,000~

*Please note that mice may not become pregnant, and pups may not be obtained by natural mating.

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